Dr Andres Floto, MB BChir, FRCP, PhD

Dr Andres FlotoHonorary Consultant in Respiratory Medicine
Research Lead, Cambridge Centre for Lung Infection
Wellcome Trust Senior Clinical Fellow & Principle Investigator
» Cambridge Institute for Medical Research

 

Contact Details
Phone 01480 364673 (sec)     Fax 01480 364330

Specialist Register
• Respiratory Medicine (adults)

Specialist interests
• Cystic Fibrosis (CF)
• Non-CF Bronchiectasis
• Lung infection (TB, Non Tuberculous Mycobacteria (NTM) and Aspergillus)
• Primary and Secondary Immunodeficiencies

Research interests
• Dendritic cell and macrophage biology
• Mycobacterial-immune cell interactions
• Pneumococcal infection and immunity
• Metabolic control of lung inflammation
• See CIMR website for more details

Professional positions
• Research Director, Cambridge Centre for Lung Infection
• Principle Investigator, Cambridge Institute for Medical Research
• Honorary Consultant, Papworth Hospital
• Honorary Consultant, Addenbrooke’s Hospital

Professional Membership
Member, British Thoracic Society
• Member, European Respiratory Society
• Member, American Thoracic Society
• Collegiate Member, Royal College of Physicians of London
• Member, Association of Physicians of Great Britain and Ireland

Education and Training
• 1991 BA University of Cambridge
• 1995 MA University of Cambridge
• 1997 PhD University of Cambridge
• 1997 MB BChir University of Cambridge
• 2000 MRCP London
• 2007 CCST Respiratory Medicine

Selected Publications

Floto RA & Haworth CS.(2011) European Respiratory Society Monograph on Bronchiectasis

Renna M, Schaffner C, Brown K, Shang S, Tamayo MH, Hegyi K, Grimsey NJ, Cusens D, Coulter S, Cooper J, Bowden AR, Newton SM, Kampmann B, Helm J, Jones A, Haworth CS, Basaraba RJ, DeGroote MA, Ordway DJ, Rubinsztein DC, Floto RA. (2011). Azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection. J Clin Invest. 121, 3554-63.

Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci. 124:469-82.

Mason MJ, Schaffner C, Floto RA, Teo QA. (2011) Constitutive Expression of a Mg2+ Inhibited K+ Current and a TRPM7-like Current in Human Erythroleukemia Cells. Am J Physiol Cell Physiol. (in press) PMID: 22135214

Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA. (2009) Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 114, 318-327

Sarkar S, Ravikumar B, Floto RA, Rubinsztein DC (2009). Rapamycin and mTOR-independent autophagy inducers. Cell Death Differ 16,:46-56

Williams A, Sarkar S, Cuddon P, Ttofi EK, Saiki S, Siddiqi FH, Jahreiss L, Fleming A, Pask D, Goldsmith P, O'Kane CJ, Floto RA & Rubinsztein DC. (2008). Novel targets for Huntington's disease in an mTOR-independent autophagy pathway. Nat Chem Biol. 4, 295-305.

Brownlie RJ, Lawlor KE, Niederer HA, Cutler AJ, Xiang Z, Clatworthy MR, Floto RA, Greaves DR, Lyons PA. & Smith KG.(2008).Distinct cell-specific control of autoimmunity and infection by FcgRIIb. J Exp Med. 205, 883-895.

Floto, R.A., Sarkar, S, Perlstein, E.O., Kampmann, B., Schreiber, S.L. & Rubinsztein, D.C. (2007). Small molecule enhancers of rapamycin-induced TOR inhibition promote autophagy, reduce toxicity in Huntington’s disease models and enhance killing of mycobacteria by macrophages. Autophagy 3, 620-622.

Martineau, A.R., Wilkinson, K.A., Newton, S.M., Floto, R.A., Norman, A.W., Skolimowska, K., Davidson, R.N., Sorensen, O.E., Kampmann, B., Griffiths, C.J. & Wilkinson, R.J. (2007). IFN-gamma and TNF-inderpendent vitamin D-inducible human suppression of mycobacteria: the role of cathelicidin LL-37. J.Immunol. 178, 7190-7198.

Newland, S.A., Macaulay, I.C., Floto, R.A., de Vet, E. C., Ouwehand, W.H., Watkins, N.A., Lyons, P.A. & Campbell, R.D. (2007). The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro. Blood. 109, 4806-4809.

Clatworthy, M.R., Willcocks, L., Urban, B.,Langhorne, J., Williams, T., Watkins, N.A., Rankin, A., Floto, R.A. & Smith, K.G.C. (2007). SLE-associated defects in the inhibitory receptor FcgRIIb reduce susceptibility to malaria. Proc Natl Acad Sci U S A. 104, 7169-7174.

Floto, R.A., MacAry, P.A., Boname, J.M., Mien, T.S., Houben, E.N.G., Pieters, J., Day, C., Oehlmann, W., Singh, M., Smith, K.G.C. & Lehner, P.J. (2006). Dendritic cell stimulation by mycobacterial HSP70 is mediated through the HIV co-receptor CCR5. Science 314, 454-458.

Floto, R.A., Clatworthy, M.R., Heilbronn, K.R., Rosner, D.R., MacAry, P.A., Rankin, A., Lehner, P.J., Ouwehand, W.H., Allen, J.M., Watkins, N.A. & Smith, K.G.C (2005). An SLE-associated polymorphism of the inhibitory receptor FcgRIIb excludes it from lipid rafts and leads to loss of function. Nature Medicine, 11, 1056-1058. Corresponding author.

Sarkar, S., Floto, R.A., Berger, Z., Imarisio, S., Cordenier, A., Pasco, M., Cook, L.J. & Rubinsztein, D.C. (2005). Lithium induces autophagy by inhibiting inositol monophosphatase. J. Cell Biol. 170, 1101-1111

Barrow, A.D., Astoul, E., Floto, R.A., Brooke, G., Ingrid A., Relou, I.A., Foad, N., Smith, K.G.C., Barclay. N., Ouwehand, W., Farndale, R.W., Alexander, D.R. & Trowsdale, J. (2004). Human TREM-like transcript-1 is a platelet specific ITIM containing co-activating receptor. J. Immunol. 172, 5838-5842.

MacAry, P.A., Javid, B., Floto, R.A., Smith, K.G.C., Singh, M. & Lehner, P.J. (2004). HSP70 Peptide-binding mutants separate antigen delivery from dendritic cell stimulation. Immunity 20, 95-106

Floto, R.A. & Smith, K.G.C. (2003). The vagus nerve, macrophages and nicotine. Lancet, 361, 1069-1070. Corresponding author.

Melendez, A.R., Bruetschy, L., Floto, R.A., Harnett, M.M. & Allen, J.M. (2001). Functional coupling of FcgRI to NADPH oxidative burst and immune complex trafficking requires the activation of phospholipase D1. Blood 98, 3421-3428.

Melendez, A.R., Floto, R.A., Gillooly, D.J., Harnett, M.M.& Allen, J.M. (1998). FcgRI-coupling to phospholipase D initiates sphingosine kinase-mediated calcium mobilisation and vesicular trafficking. J.Biol.Chem. 273, 9393-9402.

Melendez, A.R., Floto, R.A., Cameron, A.J., Gillooly, D.J., Harnett, M.M. & Allen, J.M. (1998). Molecular switch by FcgRI antibody receptor mobilises calcium through two distinct signalling pathways. Current Biology 8, 210-221.

Norman, J.C., Harrison, P.T., Davis, W., Floto, R.A. & Allen, J.M. (1998). Vesicular trafficking of FcgRI from an early endosome compartment requires recruitment of tyrosine kinases. Immunology 94, 48-55.

Floto, R.A., Somasundaram, B., Allen, J.M. & Mahaut-Smith, M.P. (1997) FcgRI activation triggers a novel Ca2+-activated monovalent cation channel in the human monocytic cell line, U937. J.Biol.Chem. 272, 4753-4758. Corresponding author.

Somasundaram, B., Mahaut-Smith, M.P. & Floto, R.A.(1996).Temperature-dependent block of capacitative Ca2+ influx in the human leukaemic cell line KU-812. J.Biol.Chem. 271, 26096-26104.

Floto, R.A., Mahaut-Smith, M.P., Allen, J.M. & Somasundaram, B.(1996).Differentiation of the human monocytic cell line, U937, results in the upregulation of the calcium release activated current, ICRAC J.Physiol. 495, 331-338. Corresponding author.

Floto, R.A., Somasundaram, B., Mahaut-Smith, M.P. & Allen, J.M. (1995). IgG-Induced Ca2+ oscillations in differentiated U937 cells: a study using laser scanning confocal microscopy and co-loaded Fluo3 and Fura-Red. Cell Calcium 18, 377-389. Corresponding author.

Hutchinson, M.J., Harrison, P.T., Floto, R.A. & Allen, J.M. (1995) Fcg receptor-mediated phagocytosis requires tyrosine kinase activity and is ligand independent Eur.J.Immunol. 25, 481-487